Covid-19, the Chinese diaspora, and the enduring legacy of racism in Peru / Covid-19, a diáspora chinesa e o legado duradouro do racismo no Peru

Abstract The coronavirus pandemic has exposed a global tendency throughout history to blame immigrants for propagating epidemics. Chinese individuals were thus targeted during past public health crises in Peru, but during the current coronavirus pandemic racist notions painting people of Chinese descent as "agents of contagion" diminished significantly. Here we examine three major epidemics (yellow fever, the bubonic plague, and covid-19) to demonstrate the current and somewhat surprising shift in negative attitudes toward the Chinese community. Peruvians' refusal to embrace derogatory terms (the "Chinese virus") or target individuals of Asian descent constitutes an intriguing case at a moment when xenophobic discourse is rampant in the Western hemisphere.

Resumo A pandemia do coronavírus expôs uma tendência mundial ao longo da história de culpar imigrantes pela propagação de epidemias. No Peru, os chineses sofreram preconceito racial durante crises de saúde pública anteriores. Surpreendentemente, a ideia de que os descendentes de chineses são "agentes de contágio" diminuiu significativamente durante a atual pandemia. Examinaremos aqui três grandes epidemias (febre amarela, peste bubônica e covid-19), a fim de demonstrar a atual mudança nas atitudes negativas em relação à comunidade chinesa. A recusa dos peruanos de adotar termos depreciativos (o "vírus chinês") ou visar indivíduos de ascendência asiática é algo intrigante em tempos de completa disseminação de discurso xenofóbico no hemisfério ocidental.

Clima organizacional en el personal de enfermería en una unidad de cuidados intensivos / Organizational environment in nursing staff in an intensive care unit

Introducción: el sistema organizacional genera un determinado clima de trabajo que repercute en las motivaciones de los miembros de la organización y en su comportamiento, por lo que al evaluar el clima organizacional se mide la forma como es percibida la organización. Objetivo: identificar el clima organizacional en el personal de enfermería de la unidad de cuidados intensivos (UCI) del Hospital de Especialidades (HE) No. 14 del Instituto Mexicano del Seguro Social (IMSS), en Veracruz, México. Metodología: estudio observacional, descriptivo y transversal, en el que participaron 49 enfermeras de los turnos matutino, vespertino y nocturno de la UCI del HE No. 14. Durante junio y julio de 2019, se aplicó el instrumento Clima Organizacional de Litwin y Stringer, que contiene 53 ítems en sus 9 dimensiones. Resultados: el nivel promedio global del clima organizacional en la UCI fue de 26.4, lo que lleva a categorizarlo como bueno. Las dimensiones: recompensa, estándares y conflicto obtuvieron una puntuación de 23.5 a 24.8, que se traduce como un promedio regular del clima organizacional. Conclusiones: hay un clima organizacional bueno en el personal de enfermería. El componente que influye de manera positiva es la dimensión cooperación, ya que el trabajador empatiza con sus compañeros de servicio. La dimensión conflicto influye de manera negativa, pues abarca la falta de motivación del personal para realizar su trabajo.

Introduction: Organizational systems generate certain workplace environment that affects the staff members' motivation and their behavior, which is why assessing the organizational environment allows to measure the staff perception on the organization. Objective: To identify the organizational environment of the nursing staff at the Specialty Hospital (SH) No. 14 intensive care unit (ICU) of the National Institute for Social Security (Instituto Mexicano del Seguro Social), in Veracruz, Mexico. Methods: Observational, descriptive, and cross-sectional study, which included 49 nurses from the morning, afternoon and night shifts of the aforementioned ICU. During June and July 2019, it was administered the instrument Organizational Climate by Litwin and Stringer, which contains 53 items in its 9 dimensions. Results: The global average level of the organizational environment in the ICU was 26.4, which leads to categorize it as good. The dimensions reward, standards and conflict obtained a range from 23.5 to 24.8, i.e., a regular average of the organizational environment. Conclusions: There is an organizational environment classified as good in the nursing staff. The component that has a positive influence is the cooperation dimension, since the worker empathizes with his fellow employees. The conflict dimension has a negative influence, given that it includes the lack of motivation of the staff to carry out their work.

Archivos digitales para historiadores: investigar en tiempos de pandemia / Digital archives for historians: research during the pandemic

Resumen Este artículo tiene como propósito poner al alcance de investigadores y estudiantes información sobre archivos digitales de bibliotecas en América Latina y en el extranjero. Su contenido está orientado para aquellos interesados en historia de la salud, con énfasis en Perú y Chile.

Abstract This article aims to provide researchers and students with information on digital archives in libraries in Latin America and elsewhere. It is aimed at those interested in health history, focusing on Peru and Chile.

Epidemias en los márgenes: la peste bubónica (1903-1905) y el covid-19 en el sur andino peruano / Epidemics on the Margins: The Bubonic Plague (1903­1905) and covid-19 Epidemics in the Peruvian South Andean Region / Epidemias marginais: a peste bubônica (1903-1905) e covid-19 no sul andino peruano

Introducción: la epidemia de covid-19 ha dejado en evidencia una serie de problemas de desigualdad en el acceso a la salud pública en Perú, especialmente en las provincias alejadas de la capital, donde se observa precariedad tanto en infraestructura como en personal sanitario. Este artículo analiza cómo el Gobierno y la población han hecho frente a epidemias en el sur de Perú, usando como casos de estudio el covid-19 y la peste bubónica ocurrida en 1903 y 1905, con el fin de entender continuidades en el manejo de la crisis epidémica y su respuesta a ellas. Desarrollo: se estudia la epidemia de la peste bubónica en las ciudades de Arequipa y Mollendo en 1903 y 1905 y el covid-19, con un enfoque en el funcionamiento del sistema de salud local y nacional, en la infraestructura sanitaria disponible para enfrentar epidemias y, finalmente, en las respuestas sociales de la población respecto al escepticismo de las medidas impuestas por las autoridades y al incremento de la automedicación. Conclusiones: la epidemia del covid-19 en el sur de Perú presenta importantes similitudes con la epidemia de peste bubónica que afectó a la región a inicios del siglo xx: desorden de responsabilidades, falta de previsión para hacer frente a enfermedades epidémicas, infraestructura precaria y una población con alto grado de desconfianza frente a las recomendaciones de las autoridades civiles y sanitarias

Introduction: The covid-19 epidemic has revealed a series of inequality problems in the access to public health services in Peru, especially in provinces far from the capital where precariousness in both infra-structure and sanitary personnel is observed. In this study, we analyzed how the population and author-ities in southern Peru faced epidemics, using covid-19 epidemic and bubonic plague in 1903 and 1905 as case studies to understand continuities in the management of epidemic crises and social response to them. Development: We studied the bubonic plague epidemic in the cities of Arequipa and Mollendo in 1903 and 1905 as well as the covid-19 epidemic, focusing on the functioning of local and national health systems, health infrastructure available to face epidemics, and finally, the social response of the population, paying particular attention to the skepticism of the population toward measures imposed by the authorities and increase in self-medication. Conclusions: The covid-19 epidemic in southern Peru pres-ents important similarities with the bubonic plague epidemic that affected the region at the beginning of the 20th century, including a disorder of responsibilities, lack of foresight to face epidemic diseases, insufficient infrastructure, and a population with a high degree of distrust in the recommendations given by the civil and health authorities

Introdução: a epidemia de covid-19 expôs uma série de problemas de desigualdade no acesso à saúde pública no Peru, especialmente nas províncias distantes da capital onde há precariedade tanto de infraestrutura quanto de pessoal de saúde. Este artigo analisa como o governo e a população têm enfren-tado epidemias no sul do Peru, utilizando o covid-19 e a peste bubônica ocorrida em 1903 e 1905 como estudos de caso, a fim de compreender as continuidades na gestão da crise epidêmica e suas respostas. Desenvolvimento: são estudadas as epidemias de peste bubônica nas cidades de Arequipa e Mollendo em 1903 e 1905 e a covid-19, enfocando no funcionamento do sistema de saúde local e nacional, a infraes-trutura de saúde disponível para enfrentar as epidemias e, por fim, as respostas da população, com particular atenção para o ceticismo em relação às medidas impostas pelas autoridades e ao aumento da automedicação. Conclusões: a epidemia de covid-19 no sul do Peru apresenta semelhanças importantes com a epidemia de peste bubônica que afetou a região no início do século XX: desordem de responsabili-dades, falta de previsão para enfrentar as doenças epidêmicas, infraestrutura precária e uma população com alto grau de desconfiança em relação às recomendações das autoridades civis e sanitárias

Las percepciones del tutor clínico sobre su desempeño docente: un estudio reflexivo / Tutorial teaching in medicine from the perspective of teachers

Background During clinical tutoring, medical students are trained to transfer theoretical knowledge to real life situations that need to be solved. Tutors should be trained to acquire skills to encourage motivation, transfer experience and inspire interest in learning among students. Aim To inquire about interests and needs during the daily work of clinical tutors. Material and Methods A qualitative study inserted in the Grounded Theory, based on the description of the meanings, in which ten clinical tutors responded to an interview in their own workplace. After obtaining consent, the responses were recorded and transcribed using Georgi's method for analysis. Results Teachers feel that there is a gap between the theoretical basis of tutorial teaching and the reality. They are especially interested in a teaching process based on values. They recognize that their pedagogical skills should be improved to improve their communication with students. They also feel that they require more skills to transfer theoretical knowledge to clinical situations. Conclusions A personal satisfaction for educating and training, the transmission of experiences, empathy and being assertive are emerging values that motivate tutors to deliver a quality education, above remunerations or contractual ties. However, the skills to transfer clinical knowledge to students should be improved.

George Deacon and the circulation of homeopathic therapies in Lima (1880-1915) / George Deacon e a circulação de tratamentos homeopáticos em Lima (1880-1915)

Abstract Homeopathy arrived from the United States to Peruvian soil in the last decades of the nineteenth century, broadening the repertoire of existing medical knowledge, which included an emerging medical profession, Chinese herbalists, and indigenous practitioners. This article examines the circulation and use of homeopathic therapies and medicines in Lima from the time when the American homeopath George Deacon initiated his practice, in the 1880s, until his death, in 1915. Although homeopathy was not the most widely used medical therapy in the country, it nevertheless posed a threat to professional medicine and the School of Medicine's desired monopoly of the field of medicine.

Resumo A homeopatia originária dos EUA adentrou solo peruano nas últimas décadas do século XIX, ampliando o repertório de conhecimento médico existente até então, o qual incluía uma profissão médica em ascensão, herbolários chineses e médicos locais. Este artigo analisa a circulação e o uso de tratamentos e medicamentos homeopáticos em Lima desde o período em que o homeopata norte-americano George Deacon iniciou sua prática, nos anos 1880, até sua morte, em 1915. Embora a homeopatia não fosse o tratamento médico mais disseminada no país, ela representou uma ameaça à medicina profissional e ao monopólio do campo da medicina almejado pela escola tradicional.

Neoliberalismo, violencia política y salud mental en Perú (1990-2006) / Neoliberalism, Political Violence and Mental Health in Peru (1990-2006) / Neoliberalismo, violência política e saúde mental no Peru (1990-2006)

Resumen Introducción: el presente artículo examina los factores que han ocasionado el precario estado de la salud mental en Perú, donde casi el 90 % de pacientes con síntomas asociados con problemas mentales no reciben tratamiento. Desarrollo: se analiza el programa neoliberal de salud implementado durante el gobierno de Alberto Fujimori (1990-2000). Posteriormente, se estudia cómo esta política pública afectó al programa de salud mental y al estado de la salud de la población. Finalmente, se examinan diversos diagnósticos sobre la salud mental posterior al retorno a la democracia en el año 2000, desde una perspectiva de los derechos humanos. Esta investigación finaliza el año 2006, momento en que el gobierno presentó un nuevo Plan Nacional de Salud Mental. Conclusiones: la falta de acceso a un tratamiento adecuado a nivel nacional ha sido consecuencia directa del reducido gasto del Estado en materia de salud, en especial hacia las enfermedades no transmisibles y de salud mental. Las políticas neoliberales de salud implementadas durante el gobierno de Alberto Fujimori redujeron el rol del Estado como garante del acceso universal a los servicios de salud, y situaron a la salud mental en un lugar marginal dentro de la salud pública. El retorno a la democracia en el año 2000, el informe final de la Comisión de la Verdad y Reconciliación (2003) y el llamado de organismos internacionales, Iglesias Católicas y Evangélicas, junto con ONG, presionaron al gobierno para situar a la salud mental como parte importante de la agenda de pública de salud.

Abstract Objective: This paper examines the elements that have caused the precarious state of mental health in Peru, where almost 90 % of patients with symptoms associated with mental disorders do not receive treatment. Development: The article analyzes the neoliberal health program implemented during the government of Alberto Fujimori (1990-2000). Subsequently, it studies how this public policy affected the mental health program and the health status of the population. Finally, it examines several diagnoses on mental health after the return of democracy in the year 2000 from a human rights perspective. This research ended in 2006 when the government presented a new National Mental Health Plan. Conclusions: The lack of access to adequate treatment at the national level has been a direct consequence of the reduced state expenditure on health, especially towards non-transmissible diseases and mental health. The neoliberal health policies implemented during the government of Alberto Fujimori reduced the role of the State as guarantor of universal access to health services and placed mental health in a marginal place within public health. The return to democracy in 2000, the final report of the Truth and Reconciliation Commission (2003), and the call of international organizations, Catholic and Evangelical Churches, together with NGOS, pressed to the government to situate mental health as a relevant part of public health agenda.

Resumo Objetivo: o presente artigo examina os fatores que têm ocasionado o precário estado da saúde mental no Peru, onde quase o 90 % de pacientes com sintomas associados com problemas mentais não recebem tratamento. Desenvolvimento: se analisa o programa neoliberal de saúde implementado durante o governo de Alberto Fujimori (1990-2000). Posteriormente estuda-se como esta política pública afetou ao programa de saúde mental e ao estado de saúde da população. Finalmente se examinam diversos diagnósticos sobre a saúde mental posterior ao retorno da democracia no ano 2000 desde uma perspectiva dos direitos humanos. Esta pesquisa finaliza no ano 2006, momento em que o governo apresentou um novo Plano Nacional de Saúde Mental. Conclusões: a falta de acesso a um tratamento adequado no nível nacional tem sido consequência direta do reduzido gasto do Estado em matéria de saúde, em especial para as doenças não transmissíveis e de saúde mental. As políticas neoliberais de saúde implementadas durante o governo de Alberto Fujimori reduziram o rol do Estado como garante do acesso universal aos serviços de saúde, e situaram à saúde mental em um lugar marginal dentro da saúde pública. O retorno à democracia no ano 2000, o informe final da Comissão da Verdade e Reconciliação (2003), e o chamado de organismos internacionais, Igrejas Católicas e Evangélicas, junto com ONGs pressionaram ao governo para situar à saúde mental como parte importante da agenda pública de saúde.

Subgingival microbiological profile of periodontitis patients in Dominican Republic

The aim of this study was to evaluate the deproteinization of primary enamel by analyzing etching pattern types, with and without the application of 5% NaOCl before acid etching with 37% H3PO4. Fifteen extracted human primary molars were randomly selected for the present in vitro study; 1mm x 1mm blocks were prepared and divided into two groups (n = 21). These groups were treated as follows: Group AAcid Etching with 37% H3PO4 gel for 15 s; Group B5% NaOCl for 60 s + Acid Etching with 37% H3PO4for 15 s. The specimens were prepared for scanning electron microscopy analysis. The images were evaluated for quality types I and II etching of the enamel surface using ImageJ software. Datasets were checked for normality by KolgomorvSmirnov test and the nonparametric unpaired MannWhitney test was applied. The mean surface area of type I and II etching pattern values was 1922.314 µm2for Group A and 3840.473 µm2Group B. We conclude that deproteinization with 5% NaOCl prior to acid etching can be used to increase the area of adhesion and the quality of the etching pattern (AU)

El objetivo del estudio fue evaluar la desproteinización del esmalte primario a través de los tipos de patrones de grabado, con y sin NaOCl 5% utilizado antes del grabado ácido con H3PO4 37%. Quince dientes primarios humanos extraídos se seleccionaron al azar para el presente estudio in vitro, se prepararon bloques de 1mm x 1 mm y se dividieron en dos grupos (n = 21). Estos grupos se trataron de la siguiente manera: Grupo A: Grabado ácido con H3PO4 37% en gel durante 15 segundos; Grupo B: NaOCl 5% durante 60 segundos + Grabado ácido con H3PO4 37% durante 15 segundos. Las muestras se prepararon para el análisis de microscopía electrónica de barrido. Las imágenes obtenidas se evaluaron principalmente por la calidad de los grabados tipo I y II de la superficie del esmalte primario, utilizando el software Image J. Los datos se analizaron en cuanto a su normalidad mediante la prueba de KolgomorvSmirnov, se utilizó pruebas no paramétricas: Prueba de MannWhitney no pareada. Como resultado, se encontró que el área de superficie media de los valores de patrón de grabado de tipo I y II para el Grupo A era 1922,314 µm2 y el Grupo B era 3840,473 µm2. Finalmente, llegamos a la conclusión de que se puede usar la desproteinización con NaOCl 5% antes del grabado ácido para aumentar el área de adhesión y la calidad del patrón de grabado (AU)

Desenmascarando a los impostores: Los médicos profesionales y su lucha contra los falsos médicos en Perú / Unmasking impostors: Professional physicians and their struggle against fake doctors in Peru

RESUMEN La caracterización de sanadores no-titulados como "charlatanes" o "impostores" ha influido notablemente en cómo han sido percibidos por la opinión pública y en las investigaciones académicas. Se creó, entonces, una división entre los médicos profesionales y aquellos que adquirieron su conocimiento de modo tradicional y no-académico. Este artículo cuestiona la supuesta división entre dichos especialistas en el campo de la salud para ofrecer un cuadro más complejo y rico de prácticas locales a partir del caso peruano. A partir, sobre todo, de correspondencia de la Facultad de Medicina de Lima y de avisos en periódicos, reconstruimos la dinámica de las autoridades médicas en sus intentos, muchas veces infructuosos, de contener y excluir a sanadores de origen asiático, europeo o local. Para ello, estudiamos dos artefactos diseñados para legitimar y monitorear a los médicos formados profesionalmente: los títulos o diplomas y las listas de graduados, predecesores de nuestros modernos documentos de identidad y bases de datos.

ABSTRACT The characterization of non-professional healers as "quacks" or "impostors" has influenced much of how such actors have been perceived by public opinion and in academic research. As a result of this, a divide has emerged between professional physicians, on the one hand, and those who acquired their knowledge in a traditional and non-academic way, on the other. This work questions the alleged divide between these two groups in the health field in order to offer a more complex and richer picture of local practices in Peru. Based mainly on correspondence from the Faculty of Medicine in Lima and newspaper ads, we reconstructed the attempts made by medical authorities to contain and exclude healers of Asian, European, or local backgrounds, many of which failed. For this reason, we studied two specific devices designed to legitimate and monitor physicians trained professionally: degrees or diplomas and lists of graduates, both of which are predecessors to our current identification cards and databases.

In vitro microbiological analysis on antibacterial, anti-inflammatory, and inhibitory action on matrix metalloproteinases-8 of commercially available chlorhexidine digluconate mouth rinses

Chlorhexidine (CHX) mouthrinses are known to have a beneficial effect in the management of periodontal disease. The present study was designed to investigate the antibacterial, anti-inflammatory, and matrix metalloproteinases-8 (MMP-8) inhibition efficacy of eight commercially available CHX mouthrinses from the Dominican Republic. Methods: The study samples are categorized into two categories, eight commercially available CHX mouthrinses were case sample group, and positive and negative controls used in the study are categorized as control sample group. Antibacterial activity of the samples was evaluated on bacterial strains obtained from American Type Culture Collection (ATCC, Rockville, MD USA) which were Porphyromonas gingivalis, Fusobacterium nucleatum, Eikenella corrodens, and Aggregatibacter actinomycetemcomitans. Results: The study samples 1, 2, 3, 5, 6, and 8 showed significant higher antibacterial efficacy and sample 4 and 7 were less effective. Samples 1, 2, 3, 5, and 6 showed higher antibacterial efficacy with no bacterial colonies formation in dilution assay method, whereas sample 8 showed smaller colonies of bacterial growth. The halo diameter found to be average in sample 8 with 13 mm, whereas sample 9 showed 12.5 + 3.48 mm, sample 1 was with a mean of 11.79 + 3.51 mm. The smaller halo diameter and minimal antibacterial activity were observed in samples 4 (mean of 3.5 + 5.95 mm) and 7 (3.5 + 7.70 mm). All eight samples showed statistically significant higher MMP-8 inhibition activity with P < 0.0001. Conclusion: Commercially available CHX digluconate mouthrinses showed the difference in plaque inhibition with 0.12 and 0.15% concentration.

Sanadores inesperados: medicina china en la era de migración global (Lima y California, 1850-1930) / Unexpected healers: Chinese medicine in the age of global migration (Lima and California, 1850-1930)

Resumen Las masivas olas de migrantes chinos que llegaron a California y Lima en la segunda mitad del siglo XIX jugaron un rol clave en la expansión de la medicina china en ambos contextos. Desde fines de la década de 1860, los herbolarios expandieron su sistema de sanación más allá de su comunidad étnica, transformando la medicina china en una de las prácticas de sanación más adoptada por la población local. Desde una perspectiva comparada, este artículo examina las divergentes trayectorias de los sanadores chinos en Perú y EEUU, así como los factores sociales y políticos que determinaron la adaptación de este conocimiento médico, foráneo, en su nuevo entorno.

Abstract The massive waves of Chinese migrants arriving in California and Lima in the second half of the nineteenth century played a crucial role in expanding Chinese medicine in both settings. From the late 1860s on, herbalists expanded their healing system beyond their ethnic community, transforming Chinese medicine into one of the healing practices most widely adopted by the local population. This article uses a comparative approach to examine the diverging trajectories of Chinese healers in Peru and the USA, as well as the social and political factors that determined how this foreign medical knowledge adapted to its new environments.

Real-time quantitative polymerase chain reaction (QPCR) for the identification and quantification of streptococcus mutans in saliva and dental biofilm in children / Técnica de reacción de polimerasa en cadena (QPCR) en tiempo real para la identificación y cuantificación de streptococcus mutans en saliva y biopelícula dentaria de niños

ABSTRACT Introduction: the objective of this study was to use real-time qPCR to identify and quantify the Streptococcus mutans species in samples of saliva and dental biofilm. Methods: 27 children were randomly chosen with the following criteria: 8 years of age, low socio-economic levels, residing in the northern metropolitan area of Santiago de Chile; they were asked to attend an appointment while fasting with no teeth brushing for at least 12 hours, in order to collect non-stimulated saliva and a pool of supragingival dental biofilm of all the mesio-vestibular sides of anterior and posterior teeth. The amount of S. mutans in the samples was quantified by qPCR using primers that amplify a fragment of the gtfB gene of S. mutans. Results: the amplification showed 98% efficiency with a fluorescence of 3.36 cycles. The melting curve presented a single maximum at the same temperature for all samples. Conclusion: the methodology allows the specific identification and quantification of gene gtfB of S. mutans in saliva and dental biofilm in a quick and reliable manner, contributing to the identification of individual cariogenic risk.

RESUMEN. Introducción: el objetivo del presente estudio consistió en implementar la técnica de qPCR en tiempo real para identificar y cuantificar la especie Streptococcus mutans en muestras de saliva y biopelícula dentaria. Métodos: se seleccionaron al azar 27 niños de 8 años de edad, de nivel socio-económico bajo del área norte de la región metropolitana de Santiago de Chile, que se citaron en ayunas y sin cepillado durante al menos 12 horas, para colectar saliva no estimulada y un pool de biopelícula dentaria supragingival de todas las caras mesio-vestibulares de dientes anteriores y posteriores. Se cuantificó la cantidad de S. mutans en las muestras mediante qPCR empleando partidores que amplifican un fragmento del gen gtfB de S. mutans. Resultados: la amplificación presentó 98% de eficiencia con delta de fluorescencia de 3,36 ciclos. La curva de fusión (melting) presentó un solo máximo a una misma temperatura para todas las muestras. Conclusión: la metodología permite la identificación y cuantificación específica del gen gtfB de S. mutans en muestras de saliva y biopelícula dentaria, de forma rápida y exacta, aportando a la determinación del riesgo cariogénico individual.

Gestão estratégica do talento na perspectiva individual e organizacional: proposta de modelo / Strategic talent management in the individual and organizational perspective: framework proposal / Gestión estratégica del talento em una perspectiva individual y organizacional: proposición de um modelo

Este ensaio teórico tem como objetivo analisar a gestão estratégica do talento no atual contexto da "nova carreira organizacional", caracterizado pela responsabilidade partilhada entre o trabalhador e a organização, como resultado das transformações do âmbito competitivo em que operam as empresas e das consequentes mutações no mundo do trabalho. É apresentado um modelo conceitual de análise, baseado em uma revisão de literatura intensiva, que pretende fazer refletir sobre as políticas, processos e práticas de gestão dos recursos humanos promovidas pela organização, ao nível da identificação, desenvolvimento e retenção, materializadas em processos de gestão de talento, em uma perspectiva de alinhamento com atitudes e comportamentos esperados dos trabalhadores. Introduz-se, também, um novo potencial indicador para a identificação de talento interno: os comportamentos moldadores. As proposições apresentadas irão permitir analisar em contexto empírico o sucesso da gestão de talento, em termos de alavancagem de desempenho individual e organizacional, verificando a sua eficácia, possibilitando pistas para processos de melhoria contínua.

This theoretical essay aims to analyze strategic talent management in the context of the "new organizational career", characterized by the shared responsibility between the worker and the organization as a result of the changes in the competitive environment in which organizations act as well as the changes in the working world. A conceptual analysis model is presented, based on intensive literature research that intends to reflect on the policies, processes, and practices of human resources management promoted by the organization to identify, develop, and retain internal talent, aligned with the attitudes and behaviors expected in workers. A new potential indicator to identify internal talent is also introduced: crafter behavior. We present some proposals that will allow us to analyze in empirical context the success of talent management, in terms of individual and organizational performance gains, verifying its effectiveness, offering help in the necessary continuous process improvement.

Este ensayo teórico tiene como objetivo analizar la gestión estratégica del talento en el actual contexto de la "nueva carrera organizacional", caracterizado por la responsabilidad compartida entre el empleado y la organización, como resultado de las transformaciones en el ámbito competitivo en el que operan las empresas y los subsequentes câmbios en el mundo del trabajo. Se presenta un modelo conceptual de análisis, basado en una revisión intensiva literatura que pretende reflexionar sobre las políticas, procesos y prácticas de gestión de recursos humanos, promovidas éstas por la organización a nivel de la identificación, desarrollo y retención, y materializadas en los procesos de gestión del talento, en alineación con la perspectiva actitudes y comportamientos que se esperan de los trabajadores. Se introduce también, un nuevo indicador potencial para la identificación de talento interno: moldeadores de conducta. Las propuestas presentadas irían permitir analizar en el contexto empírico el éxito de la gestión del talento, en términos del empuje del desempeño individual y organizacional,verificando su eficacia, posibilitando pistas para procesos de mejoria continua.

Caries-free subjects have high levels of urease and arginine deiminase activity

Objectives: This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material and Methods: Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. Results: The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects. .

¿Se considera la alimentación saludable parte de la seguridad alimentaria y nutricional?: perspectivas desde comunidades pobres de El Salvador / Is healthy eating part of food and nutrition security?: Perspectives from poor communities in El Salvador

Antecedentes: la definición de la seguridad alimentaria y nutricional (SAN) incluye la importancia de la calidad nutricional de los alimentos además de la suficiencia. Sin embargo, aún existe un énfasis en el aspecto de la suficiencia, incluso en contextos con crecientes problemas relacionados con sobrealimentación. Objetivos: describir estrategias locales para el manejo de la alimentación en la escasez de recursos; documentar perspectivas sobre la SAN en comunidades vulnerables; evaluar enlaces conceptuales entre la SAN y la alimentación saludable en estas comunidades, y comparar las definiciones locales acerca de la SAN con la política nacional sobre la SAN. Materiales y métodos: investigación cualitativa utilizando grupos focales, entrevistas informales y observaciones no estructuradas, seguida por codificación y análisis. Resultados y conclusiones: los participantes definieron la SAN como ''asegurar'' los alimentos (disponibilidad e inocuidad, incluyendo modo de producción y procesamiento de alimentos). Hubo desacuerdo entre los participantes sobre la conexión entre la SAN y la alimentación saludable. Se encontraron áreas de concordancia y desacuerdo entre la percepción local sobre la SAN y la política nacional. Es necesario entender cómo las concepciones locales evolucionan dado cambios en los alimentos disponibles localmente y la capacidad adquisitiva de poblaciones vulnerables.

Background: the definition of food and nutrition security (FNS) includes the importance of the nutritional quality of foods other than sufficiency. However, there is still an emphasis on sufficiency, even in settings with increasing problems relating to overeating. Objectives: (1) describe local coping strategies in times of resource scarcity; (2) document perspectives related to FNS in vulnerable communities; (3) evaluate the conceptual links between FNS and healthy eating in these communities and (4) compare local FNS definitions to the national discourse found in the FNS national policy. Materials and methods: qualitative research using focus groups, informal interviews and unstructured observations, followed by analytic coding. Results and conclusions: participants defined FNS as ''securing'' the food (availability and food safety, including concerns over food production and processing methods). There was disagreement among the participants regarding the connection between FNS and healthy eating. Areas of agreement and disagreement between the local discourse on national politics and SAN were found. More information is needed on how local conceptions evolve in the light of changes in the types of food available and accessible locally for vulnerable populations.

Establishing th reference range for t lymphocyes subpopulations in adults andd children from Brazil / Estabelecimento de valores de referencias para subpopulacoes de linfocitos T em adultos e criancas no Brasil

SUMMARY In Brazil, the existing reference values for T-lymphocytes subsets are based on data originated in other countries. There is no local information on normal variation for these parameters in Brazilian adults and children. We evaluated the normal variation found in blood donors from five large Brazilian cities, in different regions, and in children living in Salvador, and Rio de Janeiro. All samples were processed by flow cytometry. The results were analyzed according to region, gender, and lifestyle of blood donors. A total of 641 adults (63% males), and 280 children (58% males) were involved in the study. The absolute CD3+, and CD4+ cells count were significantly higher for females (adults and children). Higher CD4+ cell count in adults was associated with smoking, while higher CD8+ count was found among female children. Higher counts, for all T-cells subsets, were detected in blood donors from southeast / south regions while those living in the northern region had the lowest values. Individuals from midwestern and northeastern regions had an intermediate count for all these cells subsets. However, these differences did not reach statistical significance. In Brazil, gender and smoking, were the main determinants of differences in T-lymphocytes reference values. .

RESUMO Os valores de referências de linfócitos T existentes no Brasil são baseados em dados originados de outros países. Não existem dados locais da variação normal para estes parâmetros em adultos e crianças brasileiras. Avaliamos a variação normal encontrada em doadores de sangue de cinco grandes cidades brasileiras em diferentes regiões e em crianças residentes em Salvador e Rio de Janeiro. Todas as amostras foram processadas por citometria de fluxo. Os resultados foram analisados de acordo com região, gênero e estilo de vida dos doadores. Um total de 641 adultos (63% homens) e 280 crianças (58% meninos) participaram do estudo. Valores absolutos de CD3+ e CD4+ foram significantemente maiores no gênero feminino (adultos e crianças). Maiores valores de CD4+ em adultos foram associados com tabagismo, enquanto que maiores valores de CD8+ foram encontrados entre crianças do sexo feminino. Adultos das regiões sul e sudeste apresentaram maiores valores absolutos para todas as células T enquanto que adultos da região norte, apresentaram menores valores. Indivíduos residentes no nordeste e centro-oeste obtiveram contagens intermediárias para todas as populações de células T. Entretanto, estas diferenças entre as regiões, não demonstraram diferença estatística. No Brasil, gênero e tabagismo foram os principais determinantes para diferenças em valores de referências de linfócitos T. .

Oral coinfection can stress peripheral lymphocyte to inflammatory activity in leprosy

INTRODUCTION: This study evaluated the intracellular profile of interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10) and interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) from leprosy patients based on oral infections presence to determine whether these coinfections could be associated with pro-inflammatory activity in leprosy. METHODS:Leprosy patients regardless of clinical form and specific leprosy treatment (n=38) were divided into two groups: Group I - leprosy patients with oral infections (n=19), and Group II - leprosy patients without oral infections (n=19). Non-leprosy patients presenting oral infections were assigned to the control Group (n=10). Intracellular IL-2, IL-4, IL-10 and IFN-γ production was evaluated by flow cytometry (FACS) before and 7 days after controlling the oral infection in the Group I, before and 7 days after dental prophylaxis in the Group II, and during oral infection process in control Group. RESULTS: Low percentages of CD3+ lymphocytes bearing IL-2, IL-10 and IFN-γ were observed in the Group I and Group II at baseline and 7 days after therapy or prophylaxis compared to controls. Group I showed reduced percentages of IL-4 at baseline and 7 days after therapy compared to controls, or at baseline of Group II, and the Group II showed reduced percentages of CD3+ cells bearing IL-4 compared to control. An increase of the percentages of CD3+cells bearing IL-4 was observed in the Group I after the oral infections treatment. CONCLUSIONS: The occurrence of oral infections favors the intracellular cytokines expression and, probably, the inflammatory reaction operating as a stimulatory signal triggering the leprosy reactions.

Adaptación de la Escala de Afectividad Positiva y Negativa (PANAS) a la población de adultos de la ciudad de Córdoba: análisis psicométricos preliminares / Adaptation of Positive and Negative Affectivity Scale (PANAS) to adults in Cordoba city: Preliminary psychometric analysis

El afecto o emoción como tema de investigación científica ha despertado un gran interés en los últimos años, sin embargo, en nuestro medio no se cuenta con instrumentos psicométricos que permitan una medición válida y confiable. El presente trabajo tuvo por objetivo realizar una adaptación psicométrica de la Escala de Afectividad Positiva y Negativa ([PANAS], Watson, Clark & Tellegen, 1988) a la población de adultos de la ciudad de Córdoba, Argentina. Los estudios de estructura interna sugieren la existencia de dos dimensiones subyacentes homogéneas (α= 0.73; α= 0.82) que explican un 39 % de la variabilidad de la prueba. Asimismo, los estudios de correlación ítem-total, de discriminación de ítem y de contrastación de grupos resultaron semejantes a los reportados por trabajos antecedentes. En conjunto, los resultados obtenidos son promisorios y avalan el uso del PANAS en el contexto cordobés.

The affect or emotion as a topic of scientific investigation has been of great interest in recent years, however in our environment there are no psychometric instruments which allow a valid and reliable measurement. The object of this work was to create a psychometric adaptation of the Positive and Negative Affectivity Scale ([PANAS], Watson, Clark & Tellegen, 1988) for the adult population of the city of Cordoba, Argentina. The internal structure studies suggest the existence of two subjacent homogenous dimensions (α= 0.73; α= 0.82) which explain 39% of the variability of the test. Studies of the correlation item total, of item discrimination and contrasted groups resulted similar to those reported in previous works. Altogether, the results obtained are promising and support the use of PANAS in Cordoba.

Deregulated expression of A1, Bcl-2, Bcl-xL, and Mcl-1 antiapoptotic proteins and Bid, Bad, and Bax proapoptotic genes in polycythemia vera patients

Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid expression (14.4) in comparison with healthy subjects (1.0; p=0.002). Patients' leukocytes showed an A1 augmentation (7.41, p=0.001) and a reduced expression of Bax (0.19; p=0.040) and Bad (0.2; p=0.030). There was no correlation between JAK2 V617F allele burden and molecular expression. PV patients showed alterations in Bcl-2 members' expression, which may interfere with control of apoptotic machinery and contribute to disease pathogenesis.

A desregulação da apoptose parece participar da fisiopatologia da policitemia vera (PV). Este estudo avaliou a expressão das moléculas da família Bcl-2 em células hematopoéticas CD34 + e leucócitos de 12 pacientes com PV. Foram realizados: a quantificação da expressão gênica por PCR em tempo real utilizando kit Sybrgreen Quantitect, avaliação da expressão de proteínas por western-blot e detecção da mutação JAK2 V617F segundo Baxter et al. (2005). Células CD34 + dos pacientes com PV apresentaram maior expressão de A1 e Mcl-1 (mediana: 22,6 e 5,2, respectivamente) em comparação com controles (0,9 e 0,5, p = 0,004 e p = 0,020) e expressão de Bcl-2 e Bcl-xL diminuída nestes pacientes (0,18 e 1,19) em relação aos controles (1,39 e 2,01, p = 0,006 e p = 0,020). Células CD34 + dos pacientes com PV mostraram expressão elevada de bid (14,4) em comparação aos controles (1,0; p = 0,002). Leucócitos dos pacientes mostraram aumento de A1 (7,41, p = 0,001) e expressão reduzida do Bax (0,19; p = 0,04) e Bad (0,2; p = 0,030). Não houve correlação entre percentagem de alelos JAK2 V617F mutados e expressão molecular. Pacientes com PV apresentaram alterações na expressão de moléculas Bcl-2 que podem interferir no controle da apoptose e contribuir para a patogênese da doença.